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To simultaneously quantify the levels of persulfide and polysulfide sulfur, one aliquot of reaction mixture (100 L) was blocked with IAM-TPP (1 mM) for procedure A and another aliquot of the same reaction (100 L) was blocked with IAM (1 mM) for procedure B for 20 min at RT protected from light

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Intracellular GSH and GSSG levels were corrected for intracellular protein, determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) using albumin as a standard

The treatment can be repeated when benefits begin to diminish, making it a sustainable long-term option for managing bone-on-bone knee pain.