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Albert P

Protein expression Colonies of Escherichia coli BL21 (DE3) harboring expression clones encoding recombinant GSTs M4-4, P1-1 and S1-1 were grown overnight in LB medium with 100 g ml 1 ampicillin, in a shaking incubator at 200 rpm, 37 C

Previous research has shown that inflammasomes involve the sensor molecule (NLRP3), the adaptor protein ASC (apoptosis-allied speck-alike protein with a CARD domain), and pro-caspase-1 as the effector 5 , influencing IL-1 and IL-18 5,34