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About 10000 particles were picked using a Laplacian-of-Gaussian approach to build 2D templates from eleven selected motion-corrected micrographs for further template-based picking to reach more than 1.7 10 6 particles

very few hippocampal neurons exceptionally display the morphologic evidence of apoptosis, i.e., dense chromatin condensation called apoptotic bodies (Wyllie et al., 1980)