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For intracellular cytokine, staining cells were fixed with BD Cytofix Cytoperm (BD Biosciences) for 20 min at 4C, washed twice with 1x saponin buffer (0.5% (w/v) saponin [Carl Roth], 2 mM EDTA, 2% FCS, in PBS) and stained for IFN (see Table 1) overnight at 4C in 1 saponin buffer

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