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Briefly, 50 L of concentrated conditioned cell culture medium was added to 50 L of LOX working solution containing LOX substrate, horseradish peroxidase, and Amplite horseradish peroxidase substrate and incubated at 37 C for 1 h, during which fluorescence intensity was measured at Ex/Em = 540/590 nm by a fluorescence spectrophotometer

P.SoleimaniM.BakhtiarianA.KatebiM.DjahanguiriB

The key enzyme PFK1 of glucose metabolism can regulate the activity of YAP

Finally, the beads were washed twice with 75 mM NaCl in 50 mM Tris-HCl (pH 8.0), frozen in 50 l of the buffer and stored until further processing