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Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

Acta MedicaMediterranea 32:16431649 Bush AI, Tanzi RE (2008) Therapeutics for Alzheimer's disease based on the metal hypothesis

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The expression of Rec8 is essential for maintaining the connection between sister chromatids until chromosome segregation occurs

[DOI] [PubMed] [Google Scholar] 9.Boros LG, et al