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Description

They were maintained in IMDM medium (Invitrogen), and DMEM medium (Invitrogen) correspondingly, supplemented with 10% fetal bovine serum (FBS) (S711-001S, Lonsera) and 1% Penicillin-Streptomycin (SV30010, HyClone)

For phase separation 625 l water were added, vortexed and samples incubated further 10 min as above

However, it exhibits low cell membrane permeability and demonstrates substantial toxicity in animal models, thereby constraining its potential application as a therapeutic agent (Zweygarth et al., 1986)