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In 1996, I learned for the first time that many animals live longer when they are underfed, and their life span continues to increase with decreasing calorie intake, right up to the treshold of starvation

In the 36 years since, though, the horror comedy has taken on cult status, and is widely beloved by those who have come across it on home video and at midnight screenings

Second, the c-terminal amino acid of LC3 is removed by ATG4 to form LC3-I, which is further promoted by ATG7 to generate membrane-bound lipidated LC3 (LC3-I, a characteristic autophagic signature) (Xia et al., 2021