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Briefly, metabolites in plasma (100 L) were extracted with 90% methanol (900 L), and proteins were precipitated at -20 C for 2 h, followed by centrifugation for 10 min at 4000 rpm at 4 C

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The peptide activates the PI3K/Akt signaling cascade, leading to translocation of GLUT4 glucose transporters to the cell membrane and increased cellular glucose uptake

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