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There are several potential mechanisms resulting in appearance of biomarkers in urine, for instance filtration across the glomerular basement membrane, passive or active release, resorption or catabolism

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A two-gradient mobile phase (A: water + 0.1 % formic acid and B: acetonitrile + 0.1 % formic acid) and a flow rate of 0.3 mL min-1 was used

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