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334 The N-demethylation of imatinib, the key drug for patients with chronic myeloid leukemia, is also mainly mediated by CYP2C8, for which the CYP2C8*2 and CYP2C8*3 have a gain-of-function effect on imatinib while CYP2C8*4 polymorphism was opposite

Nguyen NA, Lin Z, Mohanty I, Garg N, Schmidt EW, Agarwal V

Fluorescence protease protection (FPP) assay HeLa cells were transfected with GFP tagged construct for 48 h and grown to a density of about 1-5 10 6 cells/ml (~80% confluency for a 6 cm dish)