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Membranes were washed three times for 5 min each in PBST, followed by incubation in secondary horseradish peroxidase (HRP)-conjugated antibodies

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From this array, the minimum and maximum box flow values were determined and multiplied by a compensation factor provided by the selected algorithm 98,99 , thus producing Vt, PIF and PEF values that were used to determine accepted and rejected waveforms reported as Rejection Index (non-eupneic breathing) 97

S., Hyde, T