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Scaffold-based 3D cell culture systems In scaffold-based 3D cell culture systems, ECM-like structures are introduced to provide physical support for cell adhesion, proliferation, and differentiation, typically in the form of polymer-based scaffolds or hydrogels [87]

PMID 5909486

Thus, detecting the releasement of orexin and sleep state may be a supplementary means to keep track of the AD patients progress (Liguori et al., 2020