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These two domains are adjacent to each other on the enzyme, and together form the active site of the GST where the redox reaction occurs

Furthermore, when using cholesterol hydroperoxide (ChOOH, a specific GPX4 substrate) as a stressor, compound 102 could activate GPX4 in intact cells and protect them from ChOOH toxic effects ( Supplementary Figure S12B )

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In particular, the refolding process is highly limited by the lack of reliable biophysical methods to rapidly detect the formation of correctly refolded protein

Adding more water spreads that peptide through a larger volume, so each microgram sits in more millilitres and more units of solution