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Generation and validation of Mettl9 CatD mESC cell lines CatD To generate Mettl9 CatD mESCs lines, CAS9 was guided to the third exon of the endogenous Mettl9 locus, in the region encoding the SAM binding domain, by using an sgRNA transcribed from a px459 vector (as explained before for the Mettl9 Deg )

Wang Y, Bo J, Zhao Z, Han Y, Zhang Q, Liu L

Journal of agricultural and food chemistry 2010, 58:2095-2099

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Guerreiro R, Wojtas A, Bras J, Carrasquillo M, Rogaeva E, Majounie E, et al