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Hela cells were treated with 1 M ionomycin (Sigma # I3909) for 3 h when the cells density reached to 70% confluence

10.1016/j.spinee.2017.09.002 58 Mohd IsaI

Increased ROS levels are thought to have an oncogenic effect, inducing DNA damage and chromosomal instability to activate proto-oncogenes and inactivate tumor suppressor genes (12, 13)