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Further studies showed that irisin modulates the HIF-1 and PGC-1 pathways through SIRT1, regulating lactate metabolism and mitochondrial function repair

Hemibrains of WT (n = 3) and prkn knock-out (n = 3) mice, at ~ 12 months of age respectively, were collected, weighed and homogenized in 3 v/w of KPE-X (0.1 M potassium phosphate, 5 mM EDTA, 0.1% Triton X-100, 0.6% sulfosalicylic acid, pH 7.5) using a glass Dounce homogenizer (50 passes)