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The supernatant was injected onto a 5-ml HisTrap HP column (Cytiva), and CENP-N/L complex was coeluted using 20 mM Tris, pH 7.5, 0.3 M NaCl, 5% glycerol, 5 mM BME, and 300 mM imidazole

And they run this TSH test, which is a pituitary hormone that becomes elevated

They are both peptides, thus short chains of amino acids

Patients with acute liver or multi organ failure had ta higher rate of IFI in comparison to patients with chronic liver disease (CLD) and intestinal failure, as indication for transplantation