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Draw the appropriate volume into a sterile insulin syringe

Because after gastric bypass surgery the amount of acid and essential proteins secreted by the stomach are severely decreased, preventing crucial vitamin B12 absorption

Other tests that are sometimes used to aid the diagnosis of GS are the fasting test, which involves a 24-hour calorie restriction, and administration of rifampicin (rifampicin provocation test) which cause significant increases in serum total and unconjugated bilirubin[7]

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Target cell lines were then seeded on the E-plates and measurements were recorded every 10 min for 24 h