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For example, m 6 A directs transcripts for rapid turnover via YTHDF2 or promotes efficient translation via YTHDF1 recruitment ( 6 A deposition on cytokine-receptor and checkpoint-ligand mRNAs calibrates T-cell activation thresholds, while in tumors altered m 6 A landscapes on PD-L1 and CD80 transcripts modulate immune evasion and sensitivity to checkpoint blockade ( 6 A mapping using photo-crosslinkingassisted techniques (PA-m 6 A-seq) now achieves single-nucleotide resolution, revealing context-specific m 6 A sites that correlate with patient responses to PD-1 inhibitors in melanoma ( 2.1.1.2 m 5 C: the stability booster The m 5 C modification occurs in both coding and non-coding RNAs, and plays essential roles in RNA stability and translation efficiency