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For analytical characterization, HGH Frag (176-191) is typically evaluated by orthogonal methods: reversed-phase HPLC (C18, TFA- or formic-acid modified acetonitrile/water gradients) for chromatographic purity, high-resolution mass spectrometry for molecular weight confirmation, and, where appropriate, amino-acid analysis or Edman/UPLC-MS peptide mapping for sequence verification

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