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Birc6 is associated with vulnerability of carotid atherosclerotic plaque

Methods: Sandwich ELISAs to measure the matricellular fibrosis markers thrombospondin-2 (TSP2) and thrombospondin-4 (TSP4) as well as the metabolism/inflammation/fibrosis related markers insulin-like growth factor binding protein-7 (IGFBP7), CD163 and a disintegrin and metalloprotease thrombospondin like-9 (ADAMTS9) were developed in-house using combinations of recombinantly expressed proteins as well as commercial and self-produced monoclonal and polyclonal antibodies

When indicated, cells were exposed to menadione sodium bisulfite or N -acetyl-l-cysteine (NAC

at 16 h after LPS injection

Media was replaced on the cells with 10 ml of DMEM media supplemented with 10% FBS, 1% PenStrep, and 0.6 g/ml Puromycin