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A plot of the concentration versus the time was then generated in able to find the degradation kinetic constant and calculate the half life Microsomal metabolism was also examined by the research team using the liver microsomes from male rats and an NADPH-regenerating system prepared by adding 1.7 mg/ml NADP, 7.8 mg/ml glucose 6-phosphate, and 6 U/ml glucose-6-phosphate dehydrogenase to 10 ml of 2% sodium bicarbonate

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2.4.4 Relative quantification of cytokines expression After 24 hours of in vitro stimulation RNA was extracted from PBMCs

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