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10.1016/j.intimp.2014.06.013 Int Immunopharmacol

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Sterile water has no preservative Plain sterile water contains no antimicrobial agent, so once a vial is punctured, any unused solution should be discarded rather than stored for later draws

Additionally, excessive NO can break the balance of intracellular redox and trigger inflammatory reactions, resulting in muscle tissue damage and atrophy (Kaminski and Andrade, 2001)

(A) Plaque area measured 3 days post-infection as a percentage of wild-type