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The math is the same for any reconstituted peptide, including Tirzepatide, Semaglutide, BPC-157, CJC-1295, and Ipamorelin

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Cells were washed twice with 1 PBS, trypsinized and resuspended in 3 ml of PBS and the fluorescence was immediately read on a Becton Dickinson FACScan with Cell Quest software (BD Biosciences, San Jose, USA)