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The protocols for generation and use of shRNAs and lentivirus were approved by Institutional Biosafety Committee of Kyushu university (approval number: 4-93)

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Front Microbiol 11:1308 Loira I, Morata A, Bauelos MA, Puig-Pujol A, Guamis B, Gonzlez C, Surez-Lepe JA (2018) Use of ultra-high pressure homogenization processing in winemaking: control of microbial populations in grape musts and effects in sensory quality

Another study showed that Astragaloside IV increased ATP content, stimulated nuclear translocation of -Catenin, accelerated epithelial cell proliferation, improved the destruction of actin filaments, inhibited degradation of claudin-5 and occludin, which can effectively reduce the extent of damage to the colonic mucosa (Jiang et al., 2017)