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Briefly, the cells were lysed with RIPA buffer (Sigma Aldrich) containing 150 mM NaCl, 1.0% Nonidet-P 40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, 50 mM Tris (pH 8.0), a protease inhibitor cocktail, and PhoSTOP (Roche Molecular Biochemicals, Basel, Switzerland)

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NFKB1 promoter variation implicates shear-induced NOS3 gene expression and endothelial function in prehypertensives and stage I hypertensives

SLAP Repair Surgery The surgery is performed under general anaesthetic and via a keyhole (arthroscopic) technique

3D Co-Culture Model Matrigel basement membrane matrix (Corning, Corning, NY, USA) was thawed overnight in a 4 C refrigerator