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Effect of treatment with abemaciclib and irradiation on the concentration of glutathione (GSH) content, malondialdehyde (MDA), and nitric oxide (NO) levels in the cell lysate of MCF-7 and MDA-MB-231 cell lines Figure 7 shows that abemaciclib triggered a significant increase in the levels of MDA and NO in comparison to the untreated cells of MCF-7 and MDA-MB-231 cell lines, while GSH content decreased significantly in comparison to the untreated cells of MCF-7 and MDA-MB-231 cell lines

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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator