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The first phase includes initial processing of toxins like smoke, alcohol, pesticides, drugs, etc

Protein precipitation with organic solvents can reduce matrix interference, but still a significant background persists

Superoxide accumulation was measured using the Total ROS/Superoxide Detection Kit from Enzo Life Sciences (ENZ-51010)

Researchers must balance plasma stability with intracellular release rates, control non-specific early payload release, and address aggregation issues caused by linker hydrophobicity