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During the 7-day treatment schedule, MP-13 maintained stable antinociception without tolerance development, as evidenced by the unchanged microglial cell count in the periaqueductal gray, indicating that chronic exposure did not trigger neuroinflammatory adaptationa process in which microglia become activated, release pro-inflammatory cytokines, and contribute to opioid tolerance

Similar contradictions in neuroprotection were observed with glial cell-derived neurotrophic factor (GDNF), another member of the TGFb ligand family

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Because the body continuously uses glutathione to neutralize free radicals and support detoxification, these elevated levels gradually decline as the antioxidant is consumed through normal biological processes