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Amplex-type assays report pooled H 2 O 2 and therefore integrate multiple mitochondrial and extra-mitochondrial sites, so accurate source attribution requires site-specific probes (including genetically encoded reporters targeted to matrix, IMS, or outer membrane) and carefully controlled substrate and inhibitor protocols that isolate individual electron-transfer nodes (Waldeck-Weiermair et al

Since the Raman spectra are dominated by the amide bands of the peptide backbone, similar to ORD and CD, it can be used for the analysis of protein secondary structure by finding correlations between the positions of the amide I and amide III vibrations and fraction of each secondary structural element present in globular proteins determined from X-ray crystal structures (Williams, 1986

2I) and aggressive and non-aggressive insulitis (Fig