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This work is licensed under a Creative Commons Attribution 4.0 International Licence

Although it primarily focuses on the rodent literature, a brief discussion of sex differences in the human HPA axis is also included

Individual Physiology No two biological systems are identical

At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore