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If youve shaken it vigorously, the integrity of the solution is likely compromised and it should not be used for accurate research

Methionine is Essential amino acid for the synthesis of carrier proteins, and a lack of methionine leads to a reduction in Low-density lipoprotein synthesis and therefore the inability to transport TG out of the liver, leading to the accumulation of fatty liver [115] and steatohepatitis with similar histopathological features to human NASH [116,117,118]

Possible solution B: Change tissue source: use a tissue that is known to express the protein of interest, or screen several tissues and analyze simultaneously by SDS-PAGE, to compare binding proteins from different tissues

[DOI] [PubMed] [Google Scholar] 409.Afshar Ebrahimi F., Foroozanfard F., Aghadavod E., Bahmani F., Asemi Z

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