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The samples are centrifuged (5,000 rpm, 5 min), 30 L of the resulting supernatant and 30 L of the Ellmans reagent are added to 1 mL of phosphate buffer (pH 7.8), and the optical density is measured at =412 nm and =700 nm [19]

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J Anim Sci Biotechnol 4:19 Carneiro BA, Fujii J, Brito GA, Alcantara C, Oria RB, Lima AA et al (2006) Caspase and bid involvement in Clostridium difficile toxin A-induced apoptosis and modulation of toxin A effects by glutamine and alanyl-glutamine in vivo and in vitro

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