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RNA isolation and qRTPCR Total RNA was purified using TRIzol reagent (#15596026, Invitrogen) according to the manufacturers protocol

Hence, abnormal amino acid metabolism has diverse and important roles in various cancers, and the potential impact of metabolic control and regulation in the tumor microenvironment is becoming increasingly important

Biochimica et Biophysica Acta (BBA) 1842:12401247 Wong HS, Dighe PA, Mezera V, Monternier PA, Brand MD (2017) Production of superoxide and hydrogen peroxide from specific mitochondrial sites under different bioenergetic conditions

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Estrogen-based therapies, including hormone replacement therapy (HRT) and selective estrogen receptor modulators (SERMs), have been widely studied for their potential to alleviate symptoms such as mood swings, anxiety, depression, and cognitive decline [58]