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To enhance translational relevance, recent innovations have integrated PDOs with autologous immune cell or stromal co-culture, and in vivo models such as PDO-derived xenografts (PDXs) and liver metastasis organoid models have been applied for validation

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In cholangiocarcinoma, combined BET degradation and mTOR inhibition synergistically reduce acetylation of PSAT1-associated H3K27, inducing PSAT1 downregulation and subsequent dysfunction of the serine-glycine-one-carbon (SGOC) pathway (114)

Patients are assigned to receive IFN-K immunotherapy or placebo in addition to standard treatment with immunosuppressives, antimalarials, and/or steroids