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[153] There is evidence that for elderly people, physicians often repeatedly prescribe and administer cyanocobalamin injections inappropriately, evidenced by the majority of subjects in one large study either having had normal serum concentrations or having not been tested before the injections

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2 Methods and results 2.1 Reagents and materials Primary antibodies targeting ERK (cat: #BF8004), phosphorylated ERK (cat: #AF1015), MEK (cat: #AF6385), phosphorylated MEK (cat: #AF8035), JNK (cat: #AF6318), phosphorylated JNK (cat: #AF3318), P38 (cat: #BF8015), phosphorylated P38 (cat: #AF4001), IB- (cat: #AF5002), SOD-1 (cat: #AF5144), HO-1 (cat: #AF5393), catalase (cat: #DF7545), Nrf2 (cat: #AF0639), MMP13 (cat: #AF5355), IL-6 (cat: #DF6087), cleaved-caspase 3 (cat: #AF7022), cleaved-caspase 9 (cat: #AF5240), Bcl-2 (cat: #AF6139), and IL-1 (cat: #AF5103) were procured from Jiangsu Affinity Biosciences Co., Ltd