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For the RBC separation, the blood buffy coat was washed three times with PBS (10 mM phosphate- buffered saline, pH 7.4)

Which is scientifically legitimate for hypothesis generation but insufficient for clinical recommendations

Serotonergic mechanisms regulating the GI tract: experimental evidence and therapeutic relevance

If other symptoms accompany the darkened knees, such as itching, scabs, or flaking, a person may want to talk to their doctor