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Samples were thawed at room temperature, and RNA isolation was carried out with the Tempus spin RNA isolation kit, eluted in 90 L of RNase-free water in accordance with the manufacturers protocol (Thermo Fisher, catalog number: 4380204)

Long-term actions of vector-derived nerve growth factor or brain-derived neurotrophic factor on choline acetyltransferase and Trk receptor levels in the adult rat basal forebrain