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In overdose, the increased formation of NAPQI depletes glutathione and once glutathione is depleted by more than two-thirds it covalently binds to critical cellular proteins

Bentham Science Publishers (2020), 6380

[54]Zhang X, Rui H, et al

There is a great deal of evidence suggesting that DNA methylation defects are associated with ASDs, and the role of the methylenetetrahydrofolate reductase (MTHFR) gene in folate metabolism may contribute to epigenetic mechanisms that modify complex gene expression, thus causing autism