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10.1104/pp.16.00464 Plant Physiol

Serum testosterone, follicle stimulating hormone, luteinizing hormone, estradiol and prolactin were determined by enzyme-linked immunosorbent assays (ELISA)

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CRISPR/Cas9 To generate an Eif2ak4 / genetic deficiency in ES cells, sgRNA sequences were designed using standard design tools (IDT) and cloned into the pX458 CRISPR vector (pSpCas9(BB)-2A-GFP, #48138