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After being blocked, the membranes were incubated with primary antibodies overnight at 4 C, including GPX4 (1:1 000, #DF6701, Affinity, USA), FTL (1:5 000, Cat# 10727-1-AP, Proteintech), PTGS2 (1:1 000, AF7003, Affinity, USA), and -actin (1:1 000, #4970, Cell Signaling Technology, Danvers, MA, USA), and were then incubated with secondary antibodies

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Furthermore, Hu et al (42) discovered that the downregulation of the GPX3 gene resulted in a decrease in the wound healing capacity and transmembrane migration rate of OC and colorectal cancer cells