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Numerous in vitro studies show that ITCs induce apoptosis via both intrinsic and extrinsic pathways (108)for instance, through caspase activation (109), mitochondrial dysfunction (110), and oxidative stress generation (111)

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doi:10.1016/j.fertnstert.2013.11.008

RNA extraction and qRT-PCR Total RNA was extracted by TRIzol reagent (Invitrogen) and the quality of total RNA was assessed by a Nanodrop 2000 spectrometer (Thermo Fisher Scientific)

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