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The lysates were treated with loading buffer (protein loading buffer, LI-COR Biosciences) and 100 mM DL-DTT at 80 C for 4 min

The solutions were then filtered through suitable sterilized membrane filters of 0.2 m pore size and filled into injection vials made of glass of hydrolytic class I and sealed with sterile, teflonized rubber stoppers

The hepatotoxicity is typically reversible upon drug withdrawal, supporting an immune-mediated mechanism rather than dose-dependent toxicity (244)

[DOI] [PMC free article] [PubMed] [Google Scholar] 18.Schelbert S., Schindeldecker M., Drebber U., Witzel H.R., Weinmann A., Dries V., Schirmacher P., Roth W., Straub B.K

Tritschler, Alpha-lipoic acid: Antioxidant potency against lipid peroxidation of neural tissues in vitro and implications for diabetic neuropathy, Free Radic