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Furthermore, we have shown that verapamil and its iodinated derivative NMeOHI 2 inhibited leukotriene C 4 transport by MRP1-enriched membrane vesicles in a competitive manner 12

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After cell lysis, the chromatin was fragmented into 100500 bp by Bioruptor Sonicator (Diagenode), and protein-DNA complexes were immunoprecipitated by 5 g HIF-1 antibody or 2 g anti-IgG antibody conjugated with Dynabeads Protein G (Invitrogen) mix on a rotator at 4 C overnight

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