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People with suspected type 1 diabetes, pregnant women, children, or those acutely unwell should not be diagnosed using HbA1c fasting plasma glucose or OGTT should be used instead

The highest NADPH concentration used in this reaction was 3.5-fold higher than the enzyme concentration and the flavin reduction phase does not appear to approach a limit in terms of rate or amplitude

We propose that Tyr343 may also be involved in the NADH-induced homodimerization and cFSP1 activity regulation, supporting by that Y343A and Y343F mutations reduced its NADH oxidation activity (Supplementary Table 2)