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Double-immunofluorescent histochemistry For double-immunofluorescent histochemistry, slides were incubated with Alexa Fluor 568 donkey -mouse IgG secondary antibody (Life Technologies, 1:1,000) for the detection of -synuclein and Alexa Fluor 488 donkey -rabbit IgG (H+L) Antibody for the detection of CTIF at RT for 1 h

New Engl J Med (2013) 368:157584

The beads were washed 5 times with 10 volume of ice-cold RIPA buffer, twice with 10 volume of 0.1% SDS in PBS and then resuspended in 1 volume of 0.1% SDS/PBS and incubated for 30 min at RT

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