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Description

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C., & Chiang, A

Peptides were eluted using a non-linear 85 min gradient from 1 to 99% buffer B (0.1% (v/v) acetic acid in acetonitrile) with a constant flow rate of 300 nl/min and measured using Orbitrap mass spectrometry as described 71

Nature 1996;380:221226

To screen for control sequences which are of increased strength, a recombinant library of control sequences can be introduced into a population of cells and the level of expression of a detectable marker operably linked to the control sequences determined