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Briefly, 50 L of NADPH reagent (5 mM NADPH, 42 mM GSH and 10 units/mL of GSH-Rd in 1.25 mL of distilled water) was added to 890 L of GSH-Px buffer (50 mM Tris HCl, pH 8.0, with 0.5 mM EDTA)