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Description

Pu, Y., Huang, L., Pu, M., Kang, N., Hoang, K

The role of cerebrospinal fluid hypocretin measurement in the diagnosis of narcolepsy and other hypersomnias

2011 Sep

In the jump dilution assay, after incubation of enzyme, NADPH, and inhibitor, the reactions are diluted to well below the IC 50 for the inhibitor allowing its release from the enzyme and activity is determined